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Human/Rat Beta Amyloid(40)ELISA Kit Wako

(2010/03/31 13:45)

Human/Rat Beta Amyloid(40)ELISA Kit Wako (人/大鼠Beta淀粉质40酶联免疫试剂盒)

Grade: for Immunochemistry   
Storage condition: Keep at 2-10 degrees C.

Cat. No. 294-62501
Size :96Tests
Features

These kits are designed to be used for the quantitative determination of AB in samples such as tissue culture medium, tissue homogenate, CSF and plasma.
These kits use the monoclonal antibodies that were developed by Takeda Chemicals Industries, Ltd.
BAN50: Specifically detects the N-terminal of AB
BNT77: Specifically detects the AB(11-28) of AB
BA27 : Specifically detects the C-terminal of AB 40
BC05 : Specifically detects the C-terminal of AB 42
Variation of kit1. Human beta Amyloid (1-40) ELISA Kit Wako (Wako Catalog #292-62301, 96 tests) This kit detects the quantity of Human AB (1-40). Sensitivity* : 0.12 (pM) Dynamic range : 1.0-100 (pM)2. Human beta Amyloid (1-40) ELISA Kit Wako (Wako Catalog #298-62401, 96 tests) This kit detects the quantity of Human AB (1-42). Sensitivity* : 0.08 (pM) Dynamic range : 1.0-100 (pM)3. Human/Rat beta Amyloid (40) ELISA Kit Wako (Wako Catalog #294-62501, 96 tests) This kit detects the quantity of Human or Rat (mouse) AB (x-40) with a truncated or modified N-terminus Sensitivity* : 0.25 (pM) Dynamic range : 1.0-100 (pM)4. Human/Rat beta Amyloid (42) ELISA Kit Wako (Wako Catalog #290-62601, 96 tests) This kit detects the quantity of Human or Rat (mouse) AB (x-42) with a truncated or modified N-terminus. Sensitivity* : 0.19 (pM) Dynamic range : 1.0-100 (pM)

* : The sensitivity was determined by adding two standard deviations to the mean absorbance obtained when the zero standard was assayed 24 times.

Assay Summary1. Add 100 uL of Standards and Samples and incubate in the refrigerator with plate seal overnight.2. Add 100 uL of the HRP-conjugated Antibody Solution, seal plate and refrigerate for 1 hour.3. Add 100 uL of TMB Solution, seal plate and incubate for 30 minutes at room temperature in the dark.4. Add 100 uL the Stop Solution and read the absorbance of each well at 450 nm with a microplate reader.

(uL : microliter) 
 
References

Suzuki, N., Cheung, TT., Cai, XD., Odaka, A., Otvos, L. Jr., Eckman, C., Golde, TE. and Younkin, SG.: Science, 264, 1336 (1994).
Iwatsubo, T., Odaka, A., Suzuki, N., Mizusawa, N. and Ihara, Y.: Neuron, 13, 45 (1994).
Asami-Odaka, A., Ishibashi, Y., Kikuchi, T., Kitada, C. and Suzuki, N.: Biochemistry, 34, 10272 (1995).
Fukumoto H., Tomita T., Matsunaga H., Ishibashi Y., Saido T.C. and Iwatsubo T.: Neuroreport, 10, 2965 (1999).
Scheuner, D., Eckman, C., Jensen, M., Song, X., Citron, M., Suzuki, N., Bird, TD., Hardy, J., Hutton, M., Kukull, W., Larson, E., Levy-Lahad, E., Viitanen, M., Peskind, E., Poorkaj, P., Schellenberg, G., Tanzi, R., Wasco, W., Lannfelt, L., Selkoe, D. and Younkin, S.: Nature Med., 2, 864 (1996).
Kosaka, T., Imagawa, M., Seki, K., Araki, H., Sasaki, H., Tsuji, S., Asami-Odaka, A., Fukushima, T., Imai, K. and Iwatsubo, T.: Neurology, 48, 741 (1997).
Related product

Amyloid beta Protein Immunohistostain Kit (Wako Cat#299-56701, 50 tests)
This kit stains AB 40 or AB 42 in tissue section by the monoclonal antibodies that detects the portion of C-terminal of AB.
Anti Phosphorylated alpha Synuclein, Monoclonal Antibody (Wako Cat#014-20281, 50 uL)
Specific for human alpha Synuclein including phosphorylated Ser129. (Clone No.:pSy#64)
Amyloid beta Protein (1-40), human (Wako Cat#019-18761, 1mg)
Amyloid beta Protein (1-40) (Hydrochloride), human (Wako Cat#014-18951, 1mg)
Amyloid beta Protein (1-42), human (Wako Cat#016-18771, 0.5mg)
Amyloid beta Protein (1-42) (Hydrochloride), human (Wako Cat#011-18961, 1mg)
 

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